Urine culture is performed to detect the organism that is the causative agent of UTI (urinary tract infection). During an invasive collection, technique urine is contaminated with a normal flora of urethra for this urine culture the quantitative estimation of colony count is utilized to deal with the infection.

Media:-  nutritive agar plate

Method:-Place a drop of urine in a slide & allow it to air dry. Stained slide using gram stain evaluate the slide for bacteria or PMNs.


  • In 24 hours- if no growth or colony count < 1000 then reincubate.
  • In 48 hours if the colony not considered contaminated, it can be indicative for pathogens.
  • A single colony not considered contaminated can be indicative of pathogens.
  • Growth of these or more different organism is to be considered a contaminated specimen.
  • More than 100000 (colony-forming unit) CFU/ml in pure culture may be considered pathogens.


  • Antibiotic selection by determining whether the tested bacteria are likely to be sensitive or resistance to any bacteria. The antibiotic tested were amikacin, cefotaxime, tobramycin, norfloxacin, etc.
  • Antibiotic sensitivity pattern of UTI against E-coli used for urine test in which amikacin (97.61%) was the most effective antibacterial drug.

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