Urine culture is performed to detect the organism that is the causative agent of UTI (urinary tract infection). During an invasive collection, technique urine is contaminated with a normal flora of urethra for this urine culture the quantitative estimation of colony count is utilized to deal with the infection.
Media:- nutritive agar plate
Method:-Place a drop of urine in a slide & allow it to air dry. Stained slide using gram stain evaluate the slide for bacteria or PMNs.
- In 24 hours- if no growth or colony count < 1000 then reincubate.
- In 48 hours if the colony not considered contaminated, it can be indicative for pathogens.
- A single colony not considered contaminated can be indicative of pathogens.
- Growth of these or more different organism is to be considered a contaminated specimen.
- More than 100000 (colony-forming unit) CFU/ml in pure culture may be considered pathogens.
- Antibiotic selection by determining whether the tested bacteria are likely to be sensitive or resistance to any bacteria. The antibiotic tested were amikacin, cefotaxime, tobramycin, norfloxacin, etc.
- Antibiotic sensitivity pattern of UTI against E-coli used for urine test in which amikacin (97.61%) was the most effective antibacterial drug.