CULTURE & SENSITIVITY OF URINE

Urine culture is performed to detect the organism that is the causative agent of UTI (urinary tract infection). During an invasive collection, technique urine is contaminated with a normal flora of urethra for this urine culture the quantitative estimation of colony count is utilized to deal with the infection.

Media:-  nutritive agar plate

Method:-Place a drop of urine in a slide & allow it to air dry. Stained slide using gram stain evaluate the slide for bacteria or PMNs.

Result:-

• In 24 hours- if no growth or colony count < 1000 then reincubate.
• In 48 hours if the colony not considered contaminated, it can be indicative for pathogens.
• A single colony not considered contaminated can be indicative of pathogens.
• Growth of these or more different organism is to be considered a contaminated specimen.
• More than 100000 (colony-forming unit) CFU/ml in pure culture may be considered pathogens.

Sensitivity:-

• Antibiotic selection by determining whether the tested bacteria are likely to be sensitive or resistance to any bacteria. The antibiotic tested were amikacin, cefotaxime, tobramycin, norfloxacin, etc.
• Antibiotic sensitivity pattern of UTI against E-coli used for urine test in which amikacin (97.61%) was the most effective antibacterial drug.